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KMID : 0377619670130010105
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Korean Jungang Medical Journal
1967 Volume.13 No. 1 p.105 ~ p.111
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Effect of Low Temperatures on Dysentery Phage
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Abstract
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The author has studied on the phenomena appeared when the dysentery phage is adsorbing to host bacteria at low temperature and obtained the following results. The activity of phage itself was very stable at 0°C.
The adsorbing rate of phage to host bacteria occurred at 4°C and -5°C was about 1/2 of that occurred at 337°C.
A part of phage adsorbing to host bacteria at 0°C was inactivated while the phage adsorbing to at 10°C and 20°C was almost or completely not inactivated.
When the mixture of host cell and phage is highly diluted in the broth of 37°C and 0°C respectively after pretreating of the mixture at 0°C for 5 minutes and kept continuously at 37°C and 0°C, the phage adsorption was equally stopped in both cases. The phage activity, however, was continously inactivated at 0°C while the phage is stable at 37°C.
The phage inactivation was markedly decreased by introducing of air into the mixture of phage and host cell at 0°C.
The phage adsorbed to host bacteria for 2 minutes at 37°C was almost not inactivated even though the phage is kept at 0°C.
A part of host bacteria infected by phage at low temperature was survived and the surviving rate was decreased according to increasing of temperature for phage infection.
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